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Bio-Rad Forensic DNA Fingerprinting Kit

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Online Price: €173.05
Catalogue Price: €188.80
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Using DNA as evidence, students figure out for themselves, 'Who done it?'. DNA evidence assists in criminal, missing person, mass disaster, and paternity cases. It can be used to identify a perpetrator or exonerate the innocent. Using real DNA as evidence, your students play the role of crime scene investigator. Restriction enzymes are essential tools for molecular cloning and the mapping of genes and genomes. They are also used in genetic engineering to create recombinant DNA molecules for transforming bacterial, plant, or animal cells. Restriction enzymes recognise specific double-stranded DNA sequences and cut by making two incisions, one through each of the phosphate backbones of the double helix. The chemical bonds that the enzymes cleave are easily reformed by DNA ligases, so that restriction fragments carved from the DNA of different organisms can be spliced back together, creating new hybrid organisms. With this kit, students observe the effects of two DNA restriction enzymes on a series of plasmid DNA samples. The six DNA samples in this kit are plasmids engineered to mimic the natural variations in DNA that exist between one human being and another. One DNA sample has been collected from a 'crime scene' and five samples have been obtained from various 'suspects'. Each sample is digested using a mixture of two DNA restriction enzymes generating a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by agarose gel electrophoresis and visualised using Bio-Rad's revolutionary Fast Blastª_ DNA stain. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis is used to separate and visualise DNA fragments. The unique curriculum provided in this kit guides students through the procedure of constructing a standard curve using their own gel data. They can then use their standard curve to estimate the molecular weights of the unknown DNA fragments generated by different restriction enzymes. Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the curriculum in this kit, students also have the opportunity to read plasmid maps and predict the sizes of DNA fragments from restriction enzymes digests prior to performing the lab. They can go one step further and use restriction digest maps of lambda bacteriophage genomes (provided in the kit curriculum) to design novel plasmids. In the process of doing these extension activities, students learn how restriction enzymes function and how they are used in genetic engineering. Use this kit to open the door to rich discussions about the scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering. This kit supports 32 students. Key Kit Features:
• Study DNA and restriction enzyme functions
• Use electrophoresis to visualise DNA fragments
• Construct standard curves from student data
• Make precise determinations of DNA fragment sizes
• Complete in two 45 minute sessions
NB: You will also need horizontal gel electrophoresis chambers, adjustable micropipettes (2 - 20µl, 20 - 200µl and 100 - 1,000µl), pipette tips, power supply suitable to run the electrophoresis chamber gel staining trays.
DescriptionForensic DNA Fingerprinting Kit